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. 2023 Oct 10;15(2):2264456. doi: 10.1080/19490976.2023.2264456

Figure 3.

Figure 3.

p40N120 upregulates epigenetic program in mouse intestinal epithelial cells in vitro. YAMC cells were treated with p40F, p40N180 and p40N120 at 10 or 50 ng/ml for 4 (a, c, d) or 2 (b, d) hours or TGFβ at 10 ng/ml for 15 minutes (e). (a, c) RNA was isolated from cells for RT-PCR analysis of Setd1b and Tgfb1 mRNA levels. Setd1b and Tgfb1 mRNA expression levels in control groups were set as 1. (b, e) total cellular proteins were prepared from YAMC cells for Western blot analysis using indicated antibodies. β-actin blot was used as the protein loading control. The fold changes of band intensities compared to β-actin in the same sample are shown under the blot. (d) YAMC cell culture supernatants were collected for measuring the amount of TGFβ release using ELISA. TGFβ concentration is presented in picograms per milliliter. **p < .001, *p < .01 compared the control group. #p < .01 compared to the p40F group. In A, C, and D, each symbol represents data from one independent experiment. In B, lanes were run on the same gel but were noncontiguous, as indicated by the white lines. P-SMAD2: phosphorylated; T-SMAD2: total SMAD2.