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. 2023 Oct 10;15(2):2264456. doi: 10.1080/19490976.2023.2264456

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 4. — p40N120 stimulated epigenetic responses in human intestinal epithelial cells. Human colonoids were treated with p40F, p40N180 or p40N120 at 100 ng/ml for 48 hours. (a) colonoids were fixed and stained with hematoxylin and eosin. (b, e) RNA was isolated from colonoids for RT-PCR analysis of the levels of SETD1b and TGFb1 mRNA. Expression levels of SETD1b and TGFb1 mRNA in control groups were set as 1. The mRNA expression levels of treatment groups were compared to the control group. (c, d) colonoid sections were stained using anti-H3K4me1 antibody and Cy3-labeled secondary antibody (red). Nuclei were stained with DAPI (blue). % of H3K4me1 positive cells is shown. **p < .001, *p < .01 compared the control group. ^#p < .01 compared to the p40F group. Each symbol represents data from one independent colonoid culture experiment.