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. 2023 Oct 10;15(2):2264456. doi: 10.1080/19490976.2023.2264456

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — p40N120 supplementation in early life promotes TGFβ production and Treg differentiation in the lamina propria of the colon in adult mice. Foxp3-GFP mice were treated with p40F or p40N120 from postnatal day 2 to day 21. Mice were euthanized at the age of 8 weeks. Paraffin-embedded colon tissues were used to determine H3K4me1 expression by immunohistochemistry using anti-H3K4me1 antibody and Cy3-labeled secondary antibody (red). Nuclei were stained with DAPI (blue). Images were taken using fluorescent microscope at 40X. (b) the number of H3K4me1 positive cells per crypt is shown. (c) RNA was isolated from the colonic tissues for RT-PCR analysis of the Tgfb1 mRNA expression levels. (d) lymphocytes were isolated from the lamina propria of the colon. Representative counter plot of Foxp3 and CD4 are shown. (e) the percentages of CD4⁺ Foxp3⁺ cells in total lymphocytes were shown. **p < .001, *p < .01 compared the control group. ^#p < .01 compared the p40F group. Each symbol represents data from one mouse (b and c) or from 2–3 mice (e).