Fig 1. Adsl-1(RNAi) animals can sense and respond to salt.
(A) osm-9 mutants and N2 animals exposed to empty vector (EV) control RNAi or adsl-1 RNAi were tested for osmosensation using an osmotic ring test with 4 M NaCl. Average percentage of animals that crossed the high osmolarity ring (escaped) is plotted. Each dot represents the value from a single biological replicate with 10 animals per condition. (B) EV control and adsl-1 RNAi were examined in a chemotaxis assay for attraction to 100 mM NaCl. Each dot represents the value from a single biological replicate with >120 animals per condition per replicate. Error bars represent standard error of mean (SEM). ns, nonsignificant, *** p<0.001 using one-way ANOVA with Tukey’s Test (A) or unpaired t-test (B).