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. 2023 Sep 29;19(9):e1010974. doi: 10.1371/journal.pgen.1010974

Fig 2. Adsl-1(RNAi) animal response in a gustatory plasticity assay is robust but distinct from control animals.

Fig 2

(A) Diagram of the gustatory plasticity assay (created with Biorender.com). Animals are washed off the plates with either CTX buffer or CTX buffer with 100 mM salt and left in a conical tube for 15 minutes with or without food. They are then placed on a chemotaxis assay plate for 30 minutes. (B) Gustatory plasticity assay with 100 mM sodium chloride as the conditioned stimulus. adsl-1(RNAi) animals respond robustly but distinctly from control animals. (C) Gustatory plasticity assay with 100 mM potassium chloride as the conditioned stimulus. adsl-1(RNAi) animals respond robustly but distinctly from control animals. (D) Gustatory plasticity assays in the absence or presence (hatched bars) of food. Neither adsl-1(RNAi) nor control animals alter their chemotactic response when food is included in the preincubation with salt. Each dot represents the value from a single biological replicate that included (B) 22<n<238, (C) 33<n<266, (D) 15<n<65 animals per condition per replicate. Error bars represent standard error of the mean (SEM) * p< 0.05, ** p<0.01, *** p < .0001, **** p<0.0001 using (B-C) 2-way or (D) 3-way ANOVA with Bonferroni correction.