| Antibody |
Anti-C-peptide (Rat monoclonal) |
Developmental Studies Hybridoma Bank |
Cat# GN-ID4, RRID: AB_2255626
|
IF (1:200) |
| Antibody |
Anti-Ki67 (Mouse monoclonal) |
BD Biosciences |
Cat# BD550609, RRID: AB_393778
|
IF (1:100) |
| Antibody |
Anti-Insulin (Guinea Pig polyclonal) |
Abcam |
Cat# ab7842, RRID: AB_306130
|
IF (1:400) |
| Antibody |
Anti-Glucagon (Mouse monoclonal) |
MilliporeSigma |
Cat# G2654, RRID: AB_259852
|
IF (1:10,000) |
| Antibody |
Anti-Glucagon (Rabbit monoclonal) |
Abcam |
Cat# ab92517, RRID: AB_10561971
|
IF (1:5000) |
| Antibody |
Anti-DLK1 (Rabbit polyclonal) |
Abcam |
Cat# ab21682, RRID: AB_731965
|
IF (1:1000) |
| Antibody |
Anti-GSN (Rabbit polyclonal) |
MilliporeSigma |
Cat# HPA054026, RRID: AB_2682347
|
IF (1:50) |
| Antibody |
Anti-SMIM24 (Rabbit polyclonal) |
MilliporeSigma |
Cat# HPA045046, RRID: AB_10964444
|
IF (1:50) |
| Antibody |
Anti-Guinea Pig AF594 (Donkey polyclonal) |
Jackson ImmunoResearch |
Cat# 706-586-148, RRID: AB_2340475
|
IF (1:400) |
| Antibody |
Anti-Rat AF488 (Donkey polyclonal) |
Jackson ImmunoResearch |
Cat# 712-546-153, RRID: AB_2340686
|
IF (1:400) |
| Antibody |
Anti-Mouse AF594 (Donkey polyclonal) |
Jackson ImmunoResearch |
Cat# 715-586-150, RRID: AB_2340857
|
IF (1:400) |
| Antibody |
Anti-Mouse AF488 (Donkey polyclonal) |
Jackson ImmunoResearch |
Cat# 715-545-150, RRID: AB_2340846
|
IF (1:400) |
| Antibody |
Anti-Rabbit AF594 (Donkey polyclonal) |
Jackson ImmunoResearch |
Cat# 711-586-152, RRID: AB_2340622
|
IF (1:400) |
| Antibody |
Anti-Rabbit AF488 (Donkey polyclonal) |
Jackson ImmunoResearch |
Cat# 711-545-152, RRID: AB_2313584
|
IF (1:400) |
| Biological sample (Homo sapiens) |
Primary human pancreatic islets |
Integrated Islet Distribution Program (IIDP), Prodo Laboratories Inc, ADI Islet Core |
http://iidp.coh.org; RRID: SCR_014387, https://prodolabs.com/
|
Freshly isolated |
| Chemical compound |
DA-ZP1 |
Laboratory of Amit Choudhary |
Broad Institute of MIT and Harvard |
|
| Chemical compound |
ZIGIR |
Laboratory of Wen-hong Li |
University of Texas, Dallas |
|
| Chemical compound |
NPG |
Thermo |
N7991 |
|
| Other |
DAPI, dilactate |
Sigma |
D9564 |
IF (1:6600) |
| Other |
Miami Media #1A |
Cellgro |
98-021-CV |
Islet cell culture |
| Other |
TrypLE |
Thermo Fisher Scientific |
12604-013 |
Islet cell dissociation |
| Other |
FBS |
Thermo Fisher Scientific |
10437028 |
Islet cell dissociation |
| Other |
DPBS |
Thermo Fisher Scientific |
14190250 |
Islet cell dissociation |
| Other |
D-Glucose |
Sigma-Aldrich |
G8769 |
Glucagon secretion assay |
| Other |
FFA-BSA |
Sigma-Aldrich |
3117057001 |
Glucagon secretion assay |
| Other |
DMSO |
Sigma-Aldrich |
D2650-100 |
FACS |
| Other |
Penicillin-streptomycin |
Corning |
30-002-Cl |
Islet cell culture |
| Other |
4% PFA |
Wako |
163-20145 |
Embedding islets in agar |
| Other |
Antibody diluent |
Abcam |
Ab64211 |
IF |
| Other |
Low melting agarose |
Scientific Laboratory Supplies |
NAT1030 |
Embedding islets in agar |
| Other |
TRIzol reagent |
Invitrogen |
15596026 |
RNA isolation |
| Commercial assay or kit |
Glucagon ELISA kit |
Mercodia |
10-1271-01 |
|
| Commercial assay or kit |
MycoAlert Mycoplasma Test Kit |
Lonza |
LT07-318 |
|
| Commercial assay or kit |
CellTiter-Glo Luminescent Cell Viability Assay Kit |
Promega |
G7570 |
|
| Commercial assay or kit |
ApopTag Peroxidase In Situ Apoptosis Detection Kit |
MilliporeSigma |
S7100: RRID:AB_2661855
|
|
| Commercial assay or kit |
QIAGEN RNeasy micro kit |
QIAGEN |
74004 |
|
| Software, algorithm |
Prism v.7.0 |
GraphPad Software |
http://www.graphpad.com; RRID: SCR_002798
|
|
| Software, algorithm |
Flowjo-v10 |
FlowJo Software |
http://www.flowjo.com; RRID: SCR_008520
|
|
| Software, algorithm |
ImageJ |
ImageJ Software |
https://imagej.net: RRID:SCR_003070
|
|
| Software, algorithm |
R version 4.1.0 |
R Software |
https://www.r-project.org/
|
|
