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. 2023 Feb 23;41(10):1405–1409. doi: 10.1038/s41587-023-01676-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — A 189 antibodies cocktail was used for all four mouse samples. The bright field image, spatial gene heatmap, spatial gene UMI heatmap, spatial protein heatmap and spatial protein UMI heatmap of spleen (a), colon (b), intestine (c) and kidney (d). (e) gene and gene UMI count per pixel of all four mouse samples. The box plots were derived from n = 2500 spatial pixels. The boxplot ranges from the first to the third quartile with the median value shown as the middle line, and whiskers represent 1.5× the interquartile range. (f) Protein and protein UMI count per pixel of all four mouse samples. (g) Transcriptome sequencing saturation curve of mouse spleen and human tonsil.