Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 27;622(7982):359–366. doi: 10.1038/s41586-023-06564-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, A total of 425 genes (labelled in red) out of 611 NDD genes showing expression in hSO Dlxi1/2b::eGFP⁺ cells and human postconception weeks 8–9 ganglionic eminences were included in the CRISPR screens. Among these, 354 genes are associated with ASD and 71 genes are associated with other NDDs. b, Schematic describing the interneuron generation and migration CRISPR screens. The inset highlights how hSO-derived interneurons migrate into the hCO side of the hFA. hSO were derived from the 1205-4 hiPS cell line and hCO were derived from the 2242-1 hiPS cell line. c, Volcano plot of the casTLE-estimated maximum gene perturbation effect size and associated casTLE score for the interneuron generation screen. Genes with at least two sgRNAs having effects equal to or less than −1.57 or equal to or more than +1.57, roughly twice the standard deviation (s.d.) of negative controls, were selected as candidates (red), with labels for cell cycle genes and genes that were subsequently validated. d, Volcano plot of the casTLE-estimated maximum gene perturbation effect size and associated casTLE score for the interneuron migration screen. Genes with at least two sgRNAs having effects equal to or less than −2.5 or equal to or more than +2.5, roughly twice the s.d. of negative controls were selected as candidates (red), with labels for cytoskeleton and cell migration genes and genes that were subsequently validated.