Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 27;622(7982):359–366. doi: 10.1038/s41586-023-06564-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 10 — A, Genotype analysis of genomic DNA extracted from hSO showing the PCR amplicon of LNPK. b, Representative western blotting image showing LNPK expression in LNPK KO and Cas9-CTL hSO derived from the SEC61B-mEGFP hiPS cells. GAPDH was used as a loading control. c, Quantification of LNPK abundance (n = 4, from 3 differentiations). d, Representative time-lapse sequences of SEC61B-mEGFP⁺ cells moving in a saltatory pattern in hSO assembled with an unlabeled hCO. ER displacement was detectable in the last three steps of the four in Cas9-CTL, but not in the LNPK KO. e, Percentage of SEC61B-mEGFP⁺ saltatory moving cells in the hSO side of the hFA showing ER displacement (hFA at 29–52 days after assembly. n = 15 hFA from one differentiation). * P = 0.0218, two-tailed Mann-Whitney test. f–h, Quantification of saltation length, number of saltations, and speed of movement of SEC61B-mEGFP⁺ cells in (d) (Cas9-CTL, n = 73 cells; LNPK KO, n = 68 cells). f, ** P = 0.0042, g, h **** P < 0.0001, two-tailed Mann-Whitney test. i, PCR analysis of hiPS cells showing the amplicon of ATL1. j, Representative western blotting image showing atlastin-1 abundance. GAPDH was used as a loading control. k, Quantification of atlatin-1 abundance (n = 5 from 4 differentiations). l, Schematic illustrating experimental design for performing live imaging on the hSO side of the hFA to observe saltatory migration. m, Percentage of saltatory migrating SEC61B-mEGFP⁺ cells showing ER displacement (Cas9-CTL, n = 46 cells from 12 hFA from 3 differentiations, 34 cells displayed ER displacement; ATL1 KO, n = 22 cells from 9 hFA from 2 differentiations, 8 cells displayed ER displacement; two-sided Chi-square test, ** P = 0.0029). n–p, Quantification of saltation length (n, ** P = 0.0067), number of saltations (o, P = 0.5891), and speed of movement (p, P = 0.2646) of SEC61B-mEGFP⁺ cells in (m) (Cas9-CTL, n = 46 cells, from 3 differentiations; ATL KO, n = 22 cells from 2 differentiations); two-tailed Mann-Whitney test. Data are presented as mean ± s.e.m. (c, e, f–h, k, n–p).

Source data