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. 2023 Feb 20;41(10):1424–1433. doi: 10.1038/s41587-023-01674-2

Fig. 4. Mapping interaction between gut microbiota by colony morphology analysis.

Fig. 4

a, Images of an example plate during 6 d of growth and colony identities on the plate by 16S sequencing. b, Proportion of detectable colonies at different time points compared to day 6 for each genus. Colors indicate the family-level taxonomy. c, Correlation of colony size and number of nearby colonies for two representative ASVs. A full list of correlations is provided in Supplementary Table 8. P values are calculated by one-sided Mann–Whitney U test on area of colonies with no more than one nearby colony or no less than four nearby colonies (n = 101 versus 82 for ASV-6 and 17 versus 9 for ASV-39). Definition of box-plot elements: center line: median; box limits: upper and lower 25th quartiles; whiskers: 1.5× interquartile range. d, Pairwise growth promoting and inhibiting networks between genera. Directional growth-promoting effects are shown in red sharp arrows and directional growth-inhibiting effects are shown in blue blunt arrows. Nodes represent bacterial genera and are colored by family. Node sizes are proportional to the number of isolates used in this analysis and edges widths are proportional to the significance of the interactions.