Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 21;25(10):1535–1545. doi: 10.1038/s41556-023-01229-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a–d, Identifying substrates of Cullin-RING E3 ligases: schematic representation of the GPS ORFeome library, comprising approximately 14,000 full-length, sequence-verified barcoded human ORFs (a); schematic representation of the comparative stability profiling screen using the pan-Cullin inhibitor MLN4924, where the human ORFeome library was expressed in HEK-293T cells and partitioned into six equal bins by FACS, and using the same settings and gates, the process was repeated for cells treated with MLN4924 (b); overall distribution of stability scores, comparing untreated (grey) and MLN4924-treated (red) cells (c); and 1,554 ORFs exhibited stabilization >0.5 PSI units following MLN4924 treatment (d). e,f, Assigning substrates to individual Cullin complexes: schematic representation of the barcoded sublibrary comprising the top 540 ORFs exhibiting the greatest stabilization from d (e); comparative stability profiling was performed as depicted in b to assess the stability of the library in cells expressing either an empty vector (grey) versus C-terminally truncated DN versions of Cul1 (yellow), Cul2 (light green), Cul3 (light blue), Cul4A (pink), Cul4B (purple) or Cul5 (dark green) (f). Screen profiles for four example substrates are shown. Source numerical data are available in Supplementary Tables 8–12.