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. 2023 Sep 28;25(10):1453–1464. doi: 10.1038/s41556-023-01238-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, GFP-β5(715-769) is located on the plasma membrane with some accumulations around the perinuclear region that colocalizes with Golgi apparatus marker Golgi-RFP. Scale: full-size, 10 µm; insets, 5 µm. b, When co-expressed with GFP-β5(715-769), three μHD domain-containing FCHo2 variants (RFP-FCHo2, FCHo2_ΔF-BAR-RFP, and FCHo2_ΔIDR-RFP) are redistributed to the plasma membrane and colocalize with GFP-β5(715-769) in the perinuclear region. Scale: full-size, 10 µm; insets, 5 µm. c, When co-expressed with GFP-β5(715-769), three FCHo2 variants that don’t contain μHD domain (FCHo2_ΔµHD-RFP, FCHo2_F-BAR-RFP and FCHo2_IDR-RFP) are highly diffusive in the cytosol. d, When co-expressed with the negative control GFP-β5TM, RFP-FCHo2 shows cytosolic diffusive pattern with small puncta (left) and FCHo2_µHD is highly cytosolic (right and in Fig. 4l). Scale: full-size, 10 µm; insets, 5 µm.