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. 2023 Aug 21;10(5):3077–3090. doi: 10.1002/ehf2.14492

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© 2023 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of European Society of Cardiology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Down‐regulation of miR‐124‐3p abolishes heart protective effect of hydrogen‐rich saline (HRS) induced by hypoxia/reoxygenation (H/R) injury. (A) Expression of miR‐124‐3p decreased both in vivo and in vitro after being treated with miR‐124‐3p inhibitor. (B) Cell viability of neonatal rat cardiomyocytes (NRCMs) after H/R injury was measured. (C) Lactate dehydrogenase (LDH) activity of NRCM culture medium after H/R injury was measured. (D) Caspase‐3 activity of NRCMs after H/R injury was measured. *P < 0.05, **P < 0.01, and ***P < 0.001.