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. 2023 Aug 21;10(5):3077–3090. doi: 10.1002/ehf2.14492

Figure 7.

Figure 7

Calpain1 overexpression attenuates protective effect of hydrogen‐rich saline (HRS) on cardiomyocytes. (A) Expression of calpain1 increased after being treated with Ad‐calpain1 in neonatal rat cardiomyocytes (NRCMs). (B) Cell viability of NRCMs after hypoxia/reoxygenation (H/R) injury was measured. (C) Lactate dehydrogenase (LDH) activity of NRCM culture medium after H/R injury was measured. (D) Caspase‐3 activity of NRCMs after H/R injury was measured. (E, F) Protein expressions of caspase‐3, Bcl‐2, and Bax were detected by western blot. *P < 0.05, **P < 0.01, and ***P < 0.001.