Figure 2.

Both IGSF3 mRNA and protein are expressed in the neural crest cells. (A–F) Single cell RNA sequencing analysis at E8.5 and E9.5 represented as t-SNE plots reveals the neural crest (NC) and neural tube (NT) cell clusters defined by Sox10 (B,E) and Sox2 (C,F) expression, respectively. The subclusters are assigned according to the annotations from the original analysis of the source data²³. Igsf3 is expression is restricted to the neural crest cells at E8.5 (A). At E9.5, Igsf3 is expressed predominantly by the delaminating and migrating neural crest which is identified by Sox10 with some expression in the neural tube cells (F). (G,H) Serial sections of E9.5 embryo were stained using the anti-IGSF3 and anti-SOX10 antibodies (red color). IGSF3 expression (G) is detected in the Sox10-positive (H) neural crest cells as indicated by arrows pointing to the neural crest cells that are newly migrating out of the dorsal neural tube on both sides. Scale bars: (G,H) 50 µm.