Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 11;13:17162. doi: 10.1038/s41598-023-44093-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Loss of Igsf3 results in impaired development of intestinal muscularis propria. (A,B) Small intestines collected from the P9 WT and KO pups (N = 1 for each genotype) and stained with hematoxylin and eosin (H&E). (C–F) To visualize the developing muscle layers of the small intestine the sections were stained with antibodies against αSMA (blue in (C,D), red in (E,F). (C,D) show the whole intestinal section while the panels (E,F) show the higher magnification images. (G,H) Higher magnification H&E staining from a representative sample of the small intestine from WT (G) and KO (H) P12.5 pups (N = 3 for each genotype). The arrows indicate the thickness of the muscularis externa (muscularis propria). (I) Quantification of the thickness of the developing enteric muscularis externa from P12.5 pups shows a significant reduction in the KO intestine as compared to samples from WT littermate controls. N = 3 for each genotype. Data was measured from two sections/intestine, four images/section, and 10 measurements points per image. Scale bars; (C,D) 100 µm, (G,H) 20 µm.