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. 2023 Jul 14;46(5):1832–1848. doi: 10.1007/s10753-023-01844-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — LDs accumulated in microglia after ischemic stroke a Representative immunofluorescence images co-staining neuronal-marker (NEUN), astrocyte-marker (GFAP), microglia/macrophage-marker (IBA1) and lipid droplet dye (BODIPY) within the infarct in the sham or MCAO mouse brain sections under confocal observation (scale bar. 100 µm). b Representative immunofluorescence images stained for microglia/macrophage-marker (IBA1) and lipid droplet dye (BODIPY) in the infarct regions within brain sections of sham and MCAO (3d and 7d post stroke) mice (scale bar. 50 µm). c Quantification of average LD number, LD size and the percentage of BODIPY⁺IBA1⁺ cells in all the IBA1⁺ cells. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001, by one-way ANOVA. n = 3/group. d Flow cytometry scheme sorting CD11b⁺CD45^low microglia from the right hemispheres of sham or MCAO (3d and 7d post stroke) mice. BODIPY^high microglia were further sorted from microglia. CD11b⁺CD45^low microglia were sorted from BODIPY^high cells at the same time. e Representative flow cytometry histogram showing the mean fluorescence intensity (MFI) of BODIPY in microglia isolated from sham or MCAO mice. f-h Quantitation of BODIPY MFI in microglia sorted from right hemispheres in sham or MCAO mice (3d and 7d post the surgery) f. The percentage of BODIPY^high microglia in total microglia g and the percentage of microglia among BODIPY^high cells h were quantified respectively. Data are presented as the mean ± SEM. *P < 0.05, ***P < 0.001, by one-way ANOVA. n = 3/group.