TABLE 4.

Amount of ergosterol synthesized in vitro and the IC₅₀ of ketoconazole for in vitro ergosterol biosynthesis for the various yeast transformants^a

Transformant	Amt of ergosterol synthesized (dpm)	IC50 (nM) for in vitro ergosterol biosynthesis
JL20	8,950 ± 429	22.12 ± 3.73
JL20D	3,345 ± 324	0.11 ± 0.06
YEp51/JL20D	3,178 ± 648	0.12 ± 0.04
CPR:YEp51/JL20D	9,256 ± 482	25.49 ± 4.67
CYP51/JL20D	3,542 ± 486	0.14 ± 0.05
FUS/JL20D	9,453 ± 756	200.43 ± 9.87

^aValues are means ± standard deviations.