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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Apr 12;42(4):112390. doi: 10.1016/j.celrep.2023.112390

Figure 3. Estradiol drives female APC proliferation in an ERα-dependent manner.

Figure 3.

(A) Food intake (cage average) during vehicle or E2 treatment (days 0–2 of an HFD) in female mice (n = 14–19 mice per group).

(B) Representative BrdU histograms in APCs from estradiol treatment experiment including BrdU FMO.

(C) APC proliferation after 1 week of HFD feeding in vehicle or E2 treated female mice.

(D) Expression of estrogen receptors in female APCs from RNA-seq data (n = 5 samples per group, 3 mice pooled per sample).

(E) Schematic of APC transplantation assay into female SWAT.

(F) Representative BrdU histograms in APCs from APC-ERαKO transplant experiment including BrdU FMO.

(G) APC proliferation of transplanted ERα-KO and endogenous APCs after 1 week of HFD feeding in females (n = 4 mice per group).

Statistical significance was determined by one-way ANOVA with Šidák’s tests for (A) and unpaired t tests for (C) and (F). Error bars represent mean ± SEM. ns, not significant, *p < 0.05, **p < 0.01. APCs, adipocyte precursor cells; VWAT, perigonadal fat; SWAT, inguinal subcutaneous fat; BrdU, bromodeoxyuridine; E2, 17β-estradiol; Vh, vehicle; SD, standard diet; HFD, high-fat diet; BrdU, bromodeoxyuridine; Endog, endogenous; FMO, fluorescence minus one.

See also Figure S2.