Table 3.

Frequency (%) of open clefts from two different regions of mouse brain under fast or delayed perfusion fixation conditions.

	Fast perfusion fixation	Delayed perfusion fixation
Hippocampus CA1, stratum radiatum
Exp 1	1.2% (81)	21.4% (140) p < 0.0001
Exp 2	20% (65)	30.4% (46) (not significant)
Cerebellum molecular layer
Exp 1	5.6% (36)	27.8% (79) p < 0.01
Exp 2	20.6% (102)	37.3% (48) p < 0.05

Statistical significance within each experiment analyzed by Chi-Square test of independence, and listed in each row.

(n) = number of cleft edges scored.