Antibodies |
Alexa Fluor® 700 anti-mCD8α (clone53–6.7) |
Biolegend |
Cat# 100730 |
Ultra-LEAF™ Purified anti-mouse CD3 Antibody |
Biolegend |
Cat# 100239 |
Ultra-LEAF™ Purified anti-mouse CD28 Antibody |
Biolegend |
Cat# 102115 |
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|
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Chemicals, peptides, and recombinant proteins |
MitoTracker™ green FM |
ThermoFisher |
Cat# M7514 |
MitoTracker™ red CMXRos |
ThermoFisher |
Cat# M7512 |
PEI MAX |
Polysciences |
Cat# 24765–1 |
Fixable viability Dye eFluor 506 |
eBioscience |
Cat# 65–0866-18 |
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|
|
Critical commercial assays |
CD8+ T cell isolation kit |
Stem Cell Technologies |
Cat# 19853 |
Chromium Next GEM Single Cell ATAC Kit v2 |
10x Genomics |
PN-1000406 |
Chromium Single Cell V(D)J Reagent Kit v1.1 |
10x Genomics |
PN-1000167 |
Chromium Next GEM Chip H Single Cell Kit |
10x Genomics |
PN-1000162 |
Agilent High Sensitivity DNA Kit |
Agilent Technologies |
Cat# 5067–4626 |
Mitochondria Isolation Kit for Cultured Cells |
Thermo Fisher Scientific |
Cat# 89874 |
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|
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Deposited data |
Raw and processed data |
This paper |
GEO: GSE235675
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Processed data |
This paper |
Zenodo:https://doi.org/10.5281/zenodo.8065206
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Human reference genome UCSC, GRCh38; Murine reference genome UCSC, mm10 |
University of California Santa Cruz |
https://genome.ucsc.edu/cgi-bin/hgGateway
|
Full list of public single cell datasets used in this study |
N/A |
Table S1
|
scRNA-seq data for BCC samples |
Yost et al.37
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GEO: GSE123814
|
scRNA-seq data for ESCC samples |
Zhang et al.38
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GEO: GSE160269
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scRNA-seq data for NSCLC samples |
Zeng et al.41
|
EBI: E-MTAB-6149 |
scRNA-seq data for CRC samples |
Zeng et al.41
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EBI: E-MTAB-8410 |
scRNA-seq data for MCC samples |
Zeng et al.41
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GEO: GSE117988
|
scRNA-seq data for MCC samples |
Zeng et al.41
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GEO: GSE118056
|
scRNA-seq data for LUAD samples |
Zeng et al.41
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GEO: GSE123904
|
scRNA-seq data for STAD samples |
Zeng et al.41
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GEO: GSE134520
|
scRNA-seq data for NSCLC samples |
Zeng et al.41
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GEO: GSE143423
|
scRNA-seq data for TNBC samples |
Zeng et al.41
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GEO: GSE148673
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scRNA-seq data for MIUBC samples |
Zeng et al.41
|
GEO: GSE145137
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scRNA-seq data for PDAC samples |
Zeng et al.41
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GSA: PRJCA001063 |
bulk RNA sequencing data for cancer samples |
TCGA |
https://portal.gdc.cancer.gov/
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Experimental models: Cell lines |
|
|
KrasG12D/p53ko (KP) lung cancer cells |
Dr. Esra Akbay |
N/A |
KP-mito-DsRed |
This paper |
N/A |
MC38 colon cancer cells |
ATCC |
N/A |
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|
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Experimental models: Organisms/strains |
|
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C57BL/6J |
Jackson Laboratory |
Cat# 000664 |
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|
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Recombinant DNA |
|
|
pDsRed2-Mito |
Takara |
Cat# 632421 |
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Software and algorithms |
|
|
MERCI-mtSNP and MERCI R package code |
This paper |
https://github.com/shyhihihi/MERCI
|
CytExpert |
Beckman Coulter, Inc |
https://www.beckman.com/coulter-flow-cytometers/cytoflex/cytexpert
|
FlowJo |
Tree Star Inc. |
https://www.flowjo.com/solutions/flowjo
|
GraphPad Prism software 7.0 |
GraphPad Software, Inc. |
https://graphpad.com/scientific-software/prism/
|
Python version 3.8 |
Python Software Foundation |
https://www.python.org/downloads/
|
R version 4.0.2 |
The R Foundation |
https://www.r-project.org/
|
Cell Ranger 3.1.0 |
10x Genomics |
https://10xgenomics.com/
|
Cell Ranger ATAC 2.0.0 |
10x Genomics |
https://10xgenomics.com/
|
Seurat 3.1.2 |
Seurat developers |
https://satijalab.org/seurat/articles/get_started.html
|
pysam |
pysam developers |
https://github.com/pysam-developers/pysam
|
e1071(version 1.7.4) |
N/A |
https://cran.r-project.org/web/packages/e1071/index.html
|
fgesa(version 1.16.0) |
N/A |
https://bioconductor.org/packages/release/bioc/html/fgsea.html
|
msigdbr (version 7.2.1) |
N/A |
https://cran.r-project.org/web/packages/msigdbr/index.html
|
simplifyEnrichment (version 1.0.0) |
N/A |
https://bioconductor.org/packages/release/bioc/html/simplifyEnrichment.html
|
Cytoscape (version 3.9.1) |
Cytoscape developers |
https://cytoscape.org/
|
GSVA |
Hänzelmann et al.43
|
https://bioconductor.org/packages/release/bioc/html/GSVA.html
|
BioRender |
biorender.com
|
https://www.biorender.com/
|
|
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