. 2023 Oct 4;9(10):e20621. doi: 10.1016/j.heliyon.2023.e20621

Table 1.

Primer sequence.

Name	Primer	Sequence	Size
β-actin	Forward	5‘-CACGATGGAGGGGCCGGACTCATC-3’	240bp
β-actin	Reverse	5‘-TAAAGACCTCTATGCCAACACAGT-3’	240bp
Mus TNF-α	Forward	5‘-CGTCAGCCGATTTGCTATCT-3’	206bp
Mus TNF-α	Reverse	5‘-CGGACTCCGCAAAGTCTAAG-3’	206bp
Mus TLR4	Forward	5‘-GCCCTACCAAGTCTCAGCTA-3’	165bp
Mus TLR4	Reverse	5‘-CTGCAGCTCTTCTAGACCCA-3’	165bp

18s mRNA was used as an internal control. The expressions of TLR-4 and TNF-α were calculated according to the following formula: 2-ΔΔCt = (Ct gene of interest-Ct internal control) sample A-(Ct gene of interest-Ct internal control) sample B).