FIG. 1.

Accumulation of norfloxacin in cells of V. parahaemolyticus. V. parahaemolyticus cells were grown in LB medium supplemented with 40 mM potassium lactate. Norfloxacin was added to the cell suspensions at a final concentration of 100 μM. After 15 min, CCCP was added to the suspensions at a final concentration of 100 μM. Portions were removed at the times shown, and the concentration of norfloxacin extracted from the cells was determined with a fluorescence spectrophotometer.