Fig. 5.

Copper (II) ions-doped BCP powders favored neutrophil recruitment in vivo. (A) Representative cytograms of the air pouch cell population. (B to D) Quantification of PMNs, monocytes and macrophages recruited in the mouse air pouches 6 h after injection of DPBS/DMSO (control) or BCP powders determined by flow cytometry. *p < 0.05 vs. control. @ p < 0.05 between the indicated conditions. n = 10 (for each powder condition) and n = 16 (for the control condition). (E) Representative image of Masson's trichrome-stained histological sections and (F) immunofluorescence staining (nuclei in blue and Ly6G-positive PMNs in green) of the air pouch membrane 6 h after injection of DPBS/DMSO (control) or BCP powders in suspension. Scale bar = 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)