Figure 5.

Complement factor D (CFD) and alternative complement pathway were stimulated in hHNF1A^mut/- mice

(A) mRNA levels of genes relative to three complement pathways (n = 5 for hHNF1A^−/− group, n = 7 for hHNF1A^mut/- group).

(B) Protein levels of CFD and β-ACTIN in liver (n = 5 for each group).

(C and D) Concentration of serum CFD (C), serum and hepatic complement component 3a (C3a) (D) of 20-week-old male mice (n = 6 for hHNF1A^−/− group, n = 8 for hHNF1A^mut/- group for serum CFD levels, n = 4 for hHNF1A^−/− group, n = 7 for hHNF1A^mut/- group for serum C3a level, n = 5 for hHNF1A^−/− group, n = 9 for hHNF1A^mut/- group for hepatic C3a level).

(E) Hepatic immune infiltration of 20-week-old male mice was assessed by QPCR (n = 5 for hHNF1A^−/− group, n = 6 for hHNF1A^mut/- group). All data are presented as mean ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 by an unpaired two-tailed t test. See also Figure S4.