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. 2023 Oct 11;64(10):100440. doi: 10.1016/j.jlr.2023.100440

Fig. 3.

Fig. 3

Milk lipids sustain blood glucose homeostasis for neonatal survival. A: Isogenic mating obtain offsprings with the same genotype, but lactated differently. The neonates were lactated by their own mother, namely by Cidea+/+ (normal milk, NM) and Cidea−/− (low-fat milk, LFM) females for next experiments. B: Survival rates of neonatal mice suckling NM or LFM (n = 20 per group). C: Body weights of the indicated neonates during postneonatal 32 h (n = 6). D: Representative images of neonates lactated by Cidea+/+ and Cidea−/− females at 0 (at birth) and 24 h after birth (n = 2). Scale bar represented 1 cm. E: Blood glucose levels in the neonates (n = 10). F–I: Levels of hepatic glucose (F), TAG (G), FFA (H), and glycerol (I) of neonates lactated by NM or LFM at 0, 4, 9, and 24 h after birth (n = 6). J: Liver photos and morphology of neonatal mice at 0, 4, 9, and 24 h after birth (n = 3). Oil Red O staining, and EM were performed. Scale bar represented 0.5 cm in the liver photos. Scale bar represented 50 μm in the upper row of images and 2 μm in the bottom row of images. K, L: Glucose levels of the brain (K) and heart (L) from the neonates lactated by NM and LFM at 24 h after birth, respectively (n = 6). Data are presented as mean ± s.e.m. Data were analyzed using a two-tailed Student’s t test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. EM, electron microscope; TAG, triacylglyceride.