Fig. 3.

Simulated examples of scFv protein or full IgG protein titration ELISA results of site-specific antibodies discovered by Epivolve (a) Mod1-specific scFv or IgG protein titration ELISA results from anti-mod1 discovery phage display biopanning in phase I. A typical anti-Mod1 Ab will demonstrate specific binding to the mod1 peptide, and absence of binding to the NAT-biotin-peptide, or NAT-full-length protein, or scrambled mod1 peptide. (b) and (c) are scFv or IgG protein titration ELISA results of two different clonotypes of final anti-NAT Abs: (b) a mod1-independent Ab which demonstrates binding to mod1-peptide, NAT-peptide, NAT-full-length protein, and absence of binding to scrambled Mod1 peptide; (c) a NAT-specific Ab which demonstrates binding to NAT-peptide, NAT-full-length protein, and absence of binding to mod1-peptide and scrambled Mod1 peptide