Skip to main content
. 2023 Jul 24;27(20):3065–3074. doi: 10.1111/jcmm.17872

FIGURE 5.

FIGURE 5

PI3K/AKT promoted Tp47‐induced autophagy through FOXO1. (A) The effect of the PI3K/AKT agonist 740 Y‐P and inhibitor LY294002 on the protein levels of p‐FOXO1. (B) The effect of a FOXO1 inhibitor on autophagy‐related protein levels. (C) The effect of a FOXO1 inhibitor on autophagic flux. Scale bars = 20 μm. (D) The effect of the FOXO1 inhibitor on cellular migration. Scale bars = 100 μm. One‐way anova was utilized to compare multiple groups means with one independent variable. Two‐way anova was employed to compare multiple groups means with two independent variables. Post hoc comparisons were conducted using Tukey's t‐test. *P vs. PBS, *P < 0.05, ***P < 0.001 and NS means not significant. # P vs. Tp47, # P < 0.05, ## P < 0.01, ### P < 0.001. Abbreviations: LY, LY294002; PBS, phosphate‐buffered saline.