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. 2023 Jul 24;27(20):3075–3089. doi: 10.1111/jcmm.17874

FIGURE 3.

FIGURE 3

The effect of resveratrol on ferroptosis in long‐term heart failure. (A) Quantification of the intracellular levels of SOD using the WST‐8 method, intracellular GSH levels using a GSH detection kit, intracellular lipid oxidation level determination using a lipid oxidation (MDA) detection kit (n = 24/group). (B) Western blot (WB) detection of SLC7A11 and GPX4 expression in myocardial tissues (n = 3/group). (C) Indirect immunofluorescence detection of the levels of expression of GPX4 in myocardial tissues (magnification ×20, Scale bar 50 μm). (D)Transmission electron microscopy (TEM) was used to examine mitochondrial morphology in the myocardium (Scale bar 2 μm). Sham: sham operation group; HF: heart failure group; HF + Res: heart failure + resveratrol group; KO‐HF + Res: KO‐Sirt1 heart failure + resveratrol group. Data represent the mean ± SD for 8 mice in each group, **p < 0.001.