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(A) Resolution by ion-exchange HPLC of intracellular metabolites detected in HepG2 cells exposed to 2 μM [3H]l(−)Fd4C (11 mCi/mmol). The metabolites were identified as l(−)Fd4C (I), l(−)Fd4C 5′-monophosphate (II), l(−)Fd4C 5′-diphosphate (III), and l(−)Fd4C 5′-triphosphate (IV). (B) Time course of intracellular metabolites detected in HepG2 cells after incubation with 2 μM [3H]l(−)Fd4C (11 mCi/mmol).
