Figure 5.

GER attenuated the mRNA levels of CFA-induced pro-inflammatory mediators. IA was induced by injecting 0.1 mL of CFA, and after 7 days of CFA administration, rats were treated with the abovementioned doses of MTX or tested compound (GER). On alternative days, treatments were repeated until day 35. Relative mRNA expressions of indicated genes were measured from liver homogenates. GER significantly reduced (A) NF-kB1, (B) IL-1β, (C) TNF-α, (D) COX-2, (E) mPGES-1, (F) PTGDS, and (G) MMP-1. # ≤ 0.001 (one-way ANOVA followed by the Tukey’s multiple comparison test); RT-qPCR. Significant acronyms (#) represent the comparison of treatment groups with the disease group (CFA); n = 6.