Fig. 5.

Cell proliferation dose-response curve for LIM-2405 (A), and HT-29 (A’) cells treated with atropine for 1–48 hours (hrs). The proliferation of LIM-2405, and HT-29 cell lines treated with atropine (B), 1,1-dimethyl-4 diphenylacetoxypiperidinium iodide (4-DAMP) (B’), carbachol (B”) and donepezil (B”’) for 8 h. Boyden chamber was used to determine the effect of atropine and 4-DAMP on the migration of LIM-2405 (C) and HT-29 (C’) cells for 8, 24, and 48 h. Values in A-B”’ are mean ± standard error of the mean (SEM) from at least three independent experiments. Values in C-C’ are presented as mean ± SEM from at least two independent experiments in duplicates. Two-way ANOVA followed by Tukey’s multiple comparisons test was used, and the significance value is marked with asterisks, ****p < 0.0001