Fig. 7.

Negative control labeling of LIM-2405 cells is presented in (A), control (A’), atropine (A’’), and 4-DAMP (A’’’). HT-29 negative control is displayed in (B), control (B’), atropine (B’’), and 4-DAMP (B’’’). Negative control labeling of LIM-2405 cells is presented in (C), control (C’), atropine (C’’), and 4-DAMP (C’’’). HT-29 negative control is displayed in (D), control (D’), atropine (D’’), and 4-DAMP (D’’’). The scale bar represents 50 μm. Bar graphs displaying the mean fluorescence of PD-L1 (E), PD-L2 (E’); western blot bands for LIM-2405, and HT-29 ran on the same blot with a well separating each cell line, labeled with PD-L1 and PD-L2 are shown in (F), and western blot expression intensity (G-G’). C: control, A: atropine, 4-D: 4-DAMP. Data presented as mean ± SEM. Two-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001