Fig. 3. PEG-bl-DEAEMA-co-BMA nanocarriers formulated via FNP exhibit pH responsive and membrane-destabilizing properties. (A) Number average diameter of indicated nanocarrier after incubation in PBS at indicated pH. (B) Relative degree of erythrocyte hemolysis of indicated nanocarriers at indicated pH values. (C) Schematic depicting Gal9–mCherry assay used to investigate the degree of endosomal disruption of nanocarriers. (D) Integrated pixel intensity of the Gal9–mCherry puncta per cell for indicated nanocarriers (mean ± SEM, n = 16). Analyzed via one-way ANOVA with Tukey's post-hoc. ****P < 0.0001. (E) Representative images of NCI-H358 cells expressing Gal9-YFP following overnight treatment with indicated nanocarriers displaying distinct puncta formation.