Table 3.

IC₅₀ values of hybrid CDCs tested for lysis on HeLa cells. Hybrid CDCs with PLY D1–3 were tested for lysis on the indicated cell lines at a range of 0.08 to 50 µg ml⁻¹, and IC₅₀ values indicate the concentrations required for 50 % lysis on each cell line. Full-length PLY IC₅₀ values are included for comparison. Each IC₅₀ value average with standard deviation is calculated from three experimental repeats

	IC50 on HeLa NT control cells (µg ml−1)	IC50 on HeLa hCD59 KO cells (µg ml−1)	IC50 ratio HeLa hCD59 KO : HeLa NT
PLY	15.46±4.41	17.26±5.34	1.1
PLY–ILY	1.82±0.69	nd	na
PLY–VLY	0.67±0.26	37.60±16.80	56.1
PLY–TGY3a	nd	nd	na
PLY–TGY3a_E454G	9.48±0.79	nd	na
PLY–TGY14	11.26±3.73	nd	na
PLY–INY	8.66±0.53	9.69±0.44	1.2

na, the IC50 ratio could not be calculated; nd, the CDC did not cause any cell lysis.