Fig. 1.

Interaction of plasma purified C4BP with S. aureus and L. lactis expressing SdrE. Dy488-labelled plasma purified C4BP (10 µg ml⁻¹) was incubated with S. aureus strains and L. lactis expressing SdrE. Bacterial strains were labelled using cell trace Far Red (APC-A). C4BP binding was analysed by measuring the geometric mean fluorescence intensity (gMFI) using a BD FACS Canto flow cytometer. Bars indicate the mean; data points represent three biological replicates and error bars inform the standard deviation. Statistical differences were calculated using a one-way ANOVA analysis using Dunnett’s multiple comparisons test comparing mutants to respective isogenic WT control. *P<0.05, **P<0.01,.