Fig. 2.

Protein A mediates interaction with plasma purified C4BP. (a) Illustrates the C4BP binding capacity of multiple cell wall-anchored protein transposon mutants (SdrC, SdrD, SdrE, protein A, ClfA, ClfB, FnbpA, and FnbpB) and Sbi, a non-covalently associated protein that is related to protein A. (b), (c) C4BP binding to S. aureus WT and transposon mutants of protein A and L. lactis expressing protein A. C4BP binding was analysed by measuring the geometric mean fluorescence intensity (gMFI) using a BD FACS Canto flow cytometer. Bars indicate the mean; data points represent three biological replicates and error bars inform the standard deviation. Statistical differences were calculated using a one-way ANOVA using Dunnett’s multiple comparisons test analysis comparing mutants to respective isogenic WT control. *P<0.05, ***P<0.001,.