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. 2023 Sep 5;169(9):001391. doi: 10.1099/mic.0.001391

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© 2023 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 5. — Pre-incubation of S. aureus with C4BP had no effect in limiting complement deposition. S. aureus strain JE2 or S. pyogenes strain AP1 were pre-incubated with increasing concentrations of C4BP prior to incubation in 8 % NHS. C9 deposition was analysed using goat anti-human C9 antibody and rabbit-anti-goat AF-488 secondary antibody with the geometric mean fluorescence intensity (gMFI) using a BD FACS Canto flow cytometer. Symbols represent the mean of three biological replicates and error bars inform the standard deviation. Statistical differences were calculated using a two-way ANOVA using Sidak’s multiple comparison test. **P<0.01, ****P<0.0001,.