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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Addict Neurosci. 2023 Sep 4;8:100128. doi: 10.1016/j.addicn.2023.100128

Fig. 2.

Fig. 2.

Chronic alcohol decreases calcium activity of the indirect pathway. A. Schematic of experimental timeline. B. Surgical targeting of GCaMP6s to the indirect pathway and implantation of optic fiber. C. Example of optic fiber placement within DMS in animal perfused with 10 mM CaCl2 to show GCaMP6s expression. Lever presses (D), Lever press rate (E), and rewards earned (F) during training. For D-F, plotted are means and error bars representing ± SEM. Average (SEM) calcium activity traces by trial from Air and CIE mice in relation to lever pressing (G), onset of lever press bouts (H), and first head entry following reward delivery (I). Average (SEM) calcium activity traces by per mouse session averages during lever presses (J), onset of lever press bouts (K), and first head entry following reward delivery (L). Differences between Treatment groups was evaluated using permutation testing, with dashed line indicating p < 0.05.