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. Author manuscript; available in PMC: 2023 Oct 12.
Published in final edited form as: Cell Rep. 2023 Jun 1;42(6):112577. doi: 10.1016/j.celrep.2023.112577

Figure 3. The specific activity of telomerase is unchanged in the absence of TCAB1.

Figure 3.

(A and B) Direct telomerase extension assay (300 mM KCl) of endogenous telomerase purified from parental (WT) and TCAB1 knockout (A) HeLa and (B) Halo-TERT cell lines (LC1 and LC2 loading controls, n = 2 and 3, mean).

(C) Quantification of endogenous telomerase activity in samples from TCAB1 knockout cells relative to parental controls (n = 3, mean). TKO, TCAB1 knockout.

(D and E) Direct telomerase extension assay (150 mM KCl) of telomerase purified from parental (WT) and TCAB1 knockout overexpressing TERT and TR (–Ab is a no-TERT antibody control). (D) HeLa and (E) Halo-TERT cell lines (LC1 and LC2 loading controls). TKO, TCAB1 knockout.

(F) Quantification of telomerase activity in samples from TCAB1 knockout cells relative to parental controls (n = 4–7, mean, t test).

(G) Specific activity (total activity/TR in sample; see Figure 3F) of overexpressed telomerase purified from TCAB1 knockout cells relative to parental controls normalized to 1 (n = 4–7, mean, t test).