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. Author manuscript; available in PMC: 2023 Oct 12.
Published in final edited form as: Cell Rep. 2023 Jun 1;42(6):112577. doi: 10.1016/j.celrep.2023.112577

Figure 4. Telomerase assembly is reduced in live TCAB1 knockout cells.

Figure 4.

(A) Diagramof distinct populations of TERT particles detected in control cells (three replicates of >15 cells, mean ± SD).

(B) Fraction of slow plus static 3xFLAG-HaloTag-TERT particles in control, TCAB1 knockout, and TR knockout cells (three replicates of >15 cells, mean ± SD, t test).

(C) Quantification of the fraction of TERT particles statically bound to the nucleolus in control (WT), TCAB1 knockout, and TR knockout cells (two or three replicates of >18 cells, mean).

(D) Kymographs of HaloTag-TERT particles that co-localized with nucleoli (GFP-NPM1) in control (WT), TCAB1 knockout, and TR knockout cells.