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. Author manuscript; available in PMC: 2023 Oct 12.
Published in final edited form as: Cell Rep. 2023 Jun 1;42(6):112577. doi: 10.1016/j.celrep.2023.112577

Figure 5. TCAB1 is excluded from the nucleolus.

Figure 5.

(A) Western blot probed for TCAB1 of parental and genome-edited HeLa cells expressing HaloTag-TCAB1.

(B) Maximum intensity projection of a single-molecule imaging movie of HaloTag-TCAB1 (JFX650) in cells expressing BFP-coilin and GFP-NPM1 to mark Cajal bodies and nucleoli, respectively (scale bar, 5 μm).

(C) Western blots probed with TCAB1, fibrillarin, and lamin B1 antibodies of samples of cellular fractionation experiments (left to right: input, cytoplasm, nucleus, nucleoplasm, nucleolus) from HeLa cells.

(D) Probability density functions of the step sizes derived from nuclear HaloTag-TCAB1 trajectories and the corresponding three-state model fit using the Spot-On tool (pooled data from two replicates of two independent HaloTag-TCAB1 clones, >10 cells per replicate).

(E) Kymograph of nucleolar HaloTag-TCAB1 particles over time.

(F) Live-cell fluorescence images of single 3xFLAG-HaloTag-dyskerin particles, nucleoli marked by GFP-NPM1 (scale bar, 5 μm).

(G) Kymograph of nucleolar 3xFLAG-HaloTag-dyskerin particles over time.