Skip to main content
. Author manuscript; available in PMC: 2023 Oct 12.
Published in final edited form as: Cell Rep. 2023 Jun 1;42(6):112577. doi: 10.1016/j.celrep.2023.112577

Figure 7. Analysis of the nucleolar binding dynamics of dyskerin and TR.

Figure 7.

(A) Model of 3xMS2-TR bound to MCP-mNeon-Green.

(B) Live-cell microscopy images of control and TCAB1 knockout cells expressing 3xMS2-TR, MCP-mNeonGreen, and BFP-NLS to mark the nucleolus (scale bar, 5 μm).

(C) FRAP of 3xMS2-TR within the DFC of the nucleolus marked by BFP-NLS (scale bar, 5 μm).

(D) FRAP of MS2-TR within nucleoli of TCAB1 knockout cells (n = 12 cells, mean and 95% confidence interval).

(E) Quantification of the recovery half-time from single-exponential fits of individual recovery curves (n = 12, mean).

(F) Quantification of the fraction of fluorescence recovered from single-exponential fits of individual recovery curves (n = 12 cells, mean).

(G) Model for TCAB1-mediated retention of TR in the nucleoplasm which facilitates TR assembly with TERT.