TABLE 1.

Oligonucleotide primers used for PCR amplification of the R. typhi rpoB gene

Primer name	5′ position	Sequence (5′–3′)	Annealing tempa (°C)
1fwd	5–18	GGTTTCATTAAGGG	45
1rev	520–502	GATAAAAGAATACACCTG	45
2fwd	466–488	CGGAAAGAGTAGTTGTATCACA	37
2rev	673–656	TGCTGCCTCACGGTCCT	37
3fwd	1139–1156	AGGACCGTGAGGCAGCA	37
3rev	1708–1688	CGACCGTAATGAGTAGGATG	37
4fwd	1654–1671	AGACTTTCAGCCCTTGG	50
4rev	2089–2072	CGGTTCGCATCATCATT	50
5fwd	2083–2098	TTAGAAAATGATGAT	46
5rev	2594–2577	AGGACCAAGGCGTGTAT	46
6fwd	2592–2609	AGATACACGCCTTGGTC	46
6rev	3122–3105	TTCATTCATTACATTTG	46
7fwd	3122–3139	CAAATGTAATGAATGAA	45
7rev	3889–3872	CCACCAAAATGAGACTT	45
8fwd	3839–3856	ATTCACTCTCGTTCTAT	45
8rev	4105–4088	AGCTTTACGTTGAGACA	45

^aAnnealing temperature used during PCR amplification.