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. 2023 Sep 28;14:1276014. doi: 10.3389/fimmu.2023.1276014

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Copyright © 2023 Ono, Tanaka, Myouzen, Iwasaki, Ueda, Oda, Yamamoto, Kochi and Baba

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fcrl5 upregulation causes a break in B cell anergy. (A) A representative histogram and gMFI of cell-surface IgM on B cells from MD4 Tg (n=6), MD4/Fcrl5 Tg (n=6), MD4/ML5 Tg (n=21), and MD4/ML5/Fcrl5 Tg (n=21) mice. gMFI, geometric mean fluorescence intensity. Data are pooled from three independent experiments. (B) Proliferation of CD19⁺ cells isolated from MD4 Tg (n=4), MD4/Fcrl5 Tg (n=4), MD4/ML5 Tg (n=4), and MD4/ML5/Fcrl5 Tg (n=6) mice. CD19⁺ cells are stimulated with HEL + IL-4. Data are pooled from three independent experiments. (C) Representative histograms and gMFIs of CD69, CD86, MHC-II, PD-L1, and ICOSL expression level on splenic CD19⁺ cells isolated from MD4 Tg (n=5), MD4/Fcrl5 Tg (n=4), MD4/ML5 Tg (n=20), and MD4/ML5/Fcrl5 Tg (n=20) mice after HEL stimulation. Data are pooled from three independent experiments. (D) Frequencies of CD4⁺IFN-γ⁺ cells derived from co-culture of OT-II T cells in the presence of OVA peptide with CD19⁺ cells purified from MD4 Tg (n=4–6), MD4/Fcrl5 Tg (n=3–5), MD4/ML5 Tg (n=12–19), and MD4/ML5/Fcrl5 Tg (n=12–19) mice. Data are pooled from two or three independent experiments. Statistical data are shown as mean values with s.d., and data were analyzed by one-way ANOVA with Tukey’s multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.