Table 1. Composition of the chemically-defined growth medium.

Compound	Concentration	Units	Supplier	Part Number	Stock
Base components
MOPS	40	mM	Millipore	475898	10x in H2O
K2HPO4	5	mM	Fisher	P288	10x in H2O
NaCl	0.2	mM	Fisher	S271	100x in H2O
NH4Cl	20	mM	Fisher	A649	100x in H2O
K2SO4	10	mM	Sigma	746363	50x in H2O
MgCl2.6H2O	1	mM	Fisher	BP214	100x in H2O
MnCl2.4H2O	0.05	mM	Sigma	M3634	100x in H2O
FeSO4.7H2O	0.05	mM	Sigma	F8633	100x in H2O
Amino acids
L-Alanine	14	mM	Sigma	A7627	40x in H2O
L-Arginine	0.36	mM	Sigma	A5131	200x in H2O
Glycine	3.41	mM	Sigma	G7126	200x in H2O
L-Lysine	3.59	mM	Sigma	L5626	200x in H2O
L-Proline	1.737	mM	Sigma	P0380	200x in H2O
L-Histidine	2.664	mM	Sigma	H8125	200x in H2O
L-Serine	7.374	mM	Sigma	S4500	200x in H2O
L-Threonine	2.098	mM	Sigma	T8625	200x in H2O
L-Aspartic acid	0.083	mM	Sigma	A9256	200x in 1 M HCl
L-Asparagine	4.162	mM	Sigma	A0884	200x in 1 M HCl
L-Tyrosine	1.1035	mM	Sigma	T3754	200x in 1 M NaOH
L-Cysteine-HCl	4.758	mM	Sigma	C1276	200x in H2O
L-Valine	4.268	mM	Sigma	V0500	200x in 1 M NaOH
L-Glutamic acid	1.417	mM	Sigma	G1251	200x in 1 M HCl
L-Tryptophane	1.371	mM	Sigma	T0254	200x in 1 M NaOH
L-Phenylalanine	1.513	mM	Sigma	P2126	200x in 1 M HCl
L-Glutamine	3.267	mM	Sigma	G3126	200x in 1 M NaOH
L-Leucine	1.905	mM	Sigma	L8000	2000x in 0.5 M HCl
L-Isoleucine	1.905	mM	Sigma	I2752	2000x in 0.5 M HCl
L-Methionine	0.67	mM	Sigma	M9625	200x in 0.1 M HCl
Nucleotides
Guanine	0.033	mM	Sigma	G6779	200x in 0.1 M NaOH
Uracil	0.0445	mM	Sigma	U1128	200x in 0.1 M NaOH
Xanthine	0.0325	mM	Sigma	X4002	200x in 0.1 M NaOH
Adenine	0.037	mM	Sigma	A2786	200x in 0.1 M HCl
Carbon
Glucose	125	mM	Sigma	G8270	50x in H2O
Acetate	10	mM	Fisher	S210	100x in H2O
D,L-Lactate	0.6	mM	Sigma	69785	100x in H2O
Optional
Ascorbic acid	1.4	mM	Fisher	AA3623714	100x in H2O
Lipoic acid	1	mM	Sigma	T1395	direct
Fructose	62.5 or 125	mM	Sigma	F3510	50x in H2O