Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 12;9(41):eadg3844. doi: 10.1126/sciadv.adg3844

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 4. — (A) Schematic rendering of the LSFM apparatus. LS, laser source; TL, telescope; M, mirror; DC, dichroic; BS, beam splitter; GALVO, galvo mirror; AOTF, acousto-optical tunable filters; CAM, camera; OBJ, objective. (B) A 500-μm-thick slice in PBS before (left) and after (right) TDE tissue clearing. (C) A representative slice of a middle plane (~200-μm depth) of a 500-μm-thick slice stained with CR in blue (λ_exc = 488 nm), PI in green (λ_exc = 561 nm), and NeuN in red (λ_exc = 638 nm). Vessels are visible in the blue and green channels due to the presence of autofluorescence signals. Scale bar, 1 mm. (D) Single channels of slice in (C). Scale bar, 1 mm. (E) High-resolution insets showing the different cellular markers used in the study: calretinin (CR), propidium iodide (PI), and neuronal nuclear antigen (NeuN). Scale bar, 50 μm.