Figure 5.

(A) Time-dependent inactivation of cruzain by 1.6–104 μM DEPC at pH 7.2. The lines drawn through the experimental data points were from fitting of the data to eq 2. (B) Replot of $k_{\text{obs}}\text{vs}$ [DEPC]. Fitting of the data to eq 3b results in the following values: $k_{\text{inact }}=0.23\pm 0.02{\text{min}}^{-1},K_{\text{I}}=7\pm 1\mu \text{M}$, and $k_{\text{inact }}/K_{\text{I}}=548{\text{M}}^{-1}{\text{s}}^{-1}$.