Figure 6.

Pre-steady-state time courses of cruzain-catalyzed hydrolysis of Z-FR-AMC [0.5 (red), 1.0, 1.5, 2.0, 2.5, 3.0, 3.75, 5.0. 6.25, 7.5, and 8.75 (black) μM] in ${\text{H}}_2\text{O}$ and ${\text{D}}_2\text{O}$ at 0.002–0.1 s, with the product formation shown on the same scale. The black lines drawn through the experimental data represent fitting to eq 11.