Table 1.
Cryo-EM data collection, refinement and validation statistics
| Accession codes (PDB/EMDB) | 8JO2/EMD-36453 |
| Data collection and processing | |
| Microscope/detector | Krios/K2 |
| Magnification | 165,000 |
| Voltage | 300 kV |
| Electron exposure (e-/Å2) | 57.1 |
| Defocus range (μm) | -1 to -2.2 |
| Pixel size (Å) | 0.822 |
| Symmetry imposed | C1 |
| Initial particle images (n) | 1,048,965 |
| Final particle images (n) | 219,627/99,715 (full map/local focused map) |
| Reconstruction method | Single particle reconstruction |
| Map resolution (Å) (FSC0.143) | 2.74/3.70 |
| Map resolution range (Å) | 2.0–7.5/ 2.0–8.0 |
| Map sharpening B factor (Å2) | -64/-68.8 |
| Refinement | |
| Initial model | 4S05, 6B6H and 6CA0 |
| Model composition | |
| Non-hydrogen atoms | 35,368 |
| Protein residues | 4163 |
| Nucleotides | 130 |
| B factor (Å2) | |
| Protein | 65.44 |
| Nucleotides | 139.55 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.011 |
| Bond angles (°) | 1.245 |
| Validation | |
| MolProbity score | 2.35 |
| Clashscore | 13.86 |
| Poor rotamers (%) | 2.22 |
| Ramachandran plot | |
| Favored (%) | 93.31 |
| Allowed (%) | 6.69 |
| Disallowed (%) | 0 |